Table 1.
Primers used for the expression analysis of phenylpropanoid pathway genes by using RT q-PCR [[14]]
| Gene abbreviation | Gene definition | Gen Bank accession | Unigene ID |
Primer pair |
|---|---|---|---|---|
| 5′-Forward-3′/5′-Reverse-3′ | ||||
|
STS1 |
Stilbene synthase 1 |
DO366301 |
Vvi.8 |
CGAAGCAACTAGGCATGTGT/ |
| CTCCCCAATCCAATCCTTCA | ||||
|
PAL1 |
Phenylalanine ammonialyase |
EC987386 |
Vvi.1950 |
CCGAACCGAATCAAGGACTG/ |
| GTTCCAGCCACTGAGACAAT | ||||
|
C4H1 |
Cinnamate-4-hydroxylase |
EC995763 |
Vvi.6228 |
AAAGGGTGGGCAGTTCAGTT/ |
| GGGGGGTGAAAGGAAGATAT | ||||
|
4CL1 |
4-coumarate-CoA ligase |
EC947790 |
Vvi.1251 |
CTGATGCCGCTGTTGTTTCG/ |
| GCAGGATTTTACCCGATGGA | ||||
|
CHS1 |
Chalcone synthase1 |
EC996578 |
Vvi.117 |
GTCCCAGGGTTGATTTCCAA/ |
| TCTCTTCCTTCAGACCCAGTT | ||||
| CCR2 | Cinnamoyl-CoA reductase | CF517687 | Vvi.15864 | ACAGCATGACGACTCTCTTCG/ |
| AGTGACAAGGGGTGGATTGA |