. Author manuscript; available in PMC: 2014 Mar 13.

Published in final edited form as: Chem Rev. 2012 Dec 3;113(3):2182–2204. doi: 10.1021/cr300169a

Table 1.

Dehydratases and Hydratases of the Hot Dog Superfamily with Published Structural Data.^a

Enzyme	Species	Pathway	Oligomeric State^b	Substrate(s)	Pub. Year(s)	PDB #s^c	Active Site Cocrystals^d
Arrangement A DHs
FabA	E. coli	FAS	D	(R)-3-HODec-ACP	1996	1MKA	none
FabA	E. coli	FAS	D	(R)-3-HODec-ACP	1996	1MKB	3-decynoyl-SNAC
PaFabZ	P. aeruginosa	FAS	H1	(R)-3-HO-acyl-ACPs	2004	1U1Z	none
PfFabZ	P. falciparum	FAS	H1	(R)-3-HO-acyl-ACPs	2005	1Z6B	none
					2006	1ZHG	none
					2007	2OKH	none
					2007	2OKI	none
					2011	3AZ8	NAS21
						3AZ9	NAS91
						3AZA	NAS91-10
						3AZB	NAS91-11
HpFabZ	H. pylori	FAS	H1	(R)-3-HO-acyl-ACPs	2008	2GLL	chloride
						2GLM	none
						2GLP	none
						2GLV*	none
					2008	3B7J	none
					2008	3CF8	none
						3CF9	none
						3D04	none
					2009	3ED0	none
					2009	3DOY	none
						3DOZ	none
						3DP0	none
						3DP1	none
						3DP2	none
						3DP3	none
CjFabZ	C. jejunti	FAS	H1	(R)-3-HO-acyl-ACPs	2009	3D6X	none
DEBS DH	S. erythraea	Modular PKS	D_dhC		2008	3EL6	none
CurF DH	L. majuscula	Modular PKS	D_dhC		2010	3KG6	none
CurH DH	L. majuscula	Modular PKS	D_dhC		2010	3KG7	none
CurJ DH	L. majuscula	Modular PKS	D_dhC		2010	3KG8	none
CurK DH	L. majuscula	Modular PKS	D_dhC		2010	3KG9	none
FAS DH	S. scrofa	Animal FAS	D_dhD	(R)-3-HO-acyl-ACPs	2008	2VZ8	none
FAS DH	S. scrofa	Animal FAS	D_dhD	(R)-3-HO-acyl-ACPs	2008	2VZ9	none

Arrangement J DHs & Hydratases
FAS DH	T. lanuginosus	Fungal FAS	M_th	(R)-3-HO-acyl-ACPs	2007	2UVA	none
FAS DH	S. cerevisiae	Fungal FAS	M_th	(R)-3-HO-acyl-ACPs	2007	2UV8	none
FAS DH	S. cerevisiae	Fungal FAS	M_th	(R)-3-HO-acyl-ACPs	2007	2PFF	none
PhaJ	A. caviae	PHA Biosynthesis	D		2003	1IQ6	none
MFE-2 Hydratase 2	C. tropicalis	β-Oxidation	D_dhA		2004	1PN2	none
MFE-2 Hydratase 2	C. tropicalis	β-Oxidation	D_dhA		2004	1PN4*	(R)-3-HO-Dec-CoA
MFE-2 Hydratase 2	H. sapiens	β-Oxidation	D_dhA		2005	1S9C	none
MFE-2 Hydratase 2	D. melanogaster	β-Oxidation	D_dhA		2011	3OML	none

Abbreviations used: FAS, fatty acid synthesis; PKS, polyketide synthesis; PHA, polyhydroxyalkanoate; Dec, decanoyl; CoA, coenzyme A; ACP, acyl carrier protein.

Terms defined as in Pidugu, et al.: D, dimer; H1, trimer of dimers; D_dhA, DHD dimer corresponding to a face-to-face dimer of dimers (TA). Terms introduced in this review: D_dhC & D_dhD, DHD side-by-side dimers; M_th, THD monomer.

An asterisk indicates a mutant.

Buffers and/or salt ions are not necessarily noted nor are inhibitors not bound within the active site. The NAS compounds are a series of competitive inhibitors designed for PfFabZ.