Table 3.
Thioesterases of the Hot Dog Superfamily with Published Structural Data.a
| Enzyme | Species | Pathway | Oligomeric Stateb | Substrate(s) | Pub. Year(s) | PDB #sc | Active Site Cocrystalsd |
|---|---|---|---|---|---|---|---|
| Arrangement Ψ | |||||||
| 4HBT-I | Pseudomonas sp. strain CBS-3 | 4-HOBz Degradation | TA | 4-HOBz-CoA | 1998 | 1BVQ | none |
| 2002 | 1LO7* | 4-HOBz-CoA | |||||
| 1LO8 | 4-HOBn-CoA | ||||||
| 1LO9 | 4-HOPa-CoA | ||||||
| YbgC | H. pylori | Tol-Pal | TA | LC acyl-CoAs | 2008 | 2PZH | none |
|
| |||||||
| Arrangement R | |||||||
| 4HBT-II | Arthrobacter sp. strain SU | 4-HOBz Degradation | TB | 4-HOBz-CoA | 2003 | 1Q4S | 4-HOBz & CoA |
| 1Q4T | 4-HOBn-CoA | ||||||
| 1Q4U | 4-HOPa-CoA | ||||||
| EntH | E. coli | NRPS | TB | HOBz-PCPs | 2009 | 1VH9 | none |
| TtPaaI | T. thermophilus HB8 | PhAcOH Degradation | TB | PhAc-CoAs | 2005 | 1J1Y | none |
| 2005 | 1WLU | glycerol | |||||
| 1WLV | CoA | ||||||
| 1WM6 | none | ||||||
| 1WN3 | Hex-CoA | ||||||
| EcPaaI | E. coli | PhAcOH Degradation | TB | PhAc-CoAs | 2006 | 2FS2 | none |
| THEM2 | H. sapiens | unknown | TB | M/LC acyl-CoAs | 2006 | 2F0X | sulfate |
| 2009 | 3F5O | 2-oxoundecyl-CoA | |||||
| YciA | H. influenza | unknown | H2 | acyl-CoAs | 2008 | 1YLI | none |
| 3BJK | CoA | ||||||
| Cj0915 | C. jejuni | unknown | [H2] | acyl-CoAs | 2009 | 3D6L | none |
| Acot7 | M. musculus | unknown | Trdh | arachidonoyl-CoA | 2007 | 2V1Oe | CoA |
| 2Q2Bf | none | ||||||
| TE-II | E. coli | FAS | DdhB | MC acyl-CoA | 2000 | 1C8U | none |
|
| |||||||
| FlK | S. cattleya | FAc Detoxification | D | FAc-CoA | 2010 | 3KX7 | none |
| 3KX8 | none | ||||||
| 3KV7 | AcOH | ||||||
| 3KV8 | FAcOH | ||||||
| 3KVZ | FAc-CNAC | ||||||
| 3KW1 | FAc-ONAC | ||||||
| 3KUV* | AcOH | ||||||
| 3KUW* | FAcOH | ||||||
| 3KVU* | Ac-CoA | ||||||
| 3KVI* | FAcOH | ||||||
| 2010 | 3P2Q | none | |||||
| 3P2R | FAcOH | ||||||
| 3P2S | none | ||||||
| 3P3F* | none | ||||||
| 3P3I* | FAcOH & CoA | ||||||
| CalE7g | M. echinospora | IPKS | TA | unconfirmed | 2009 | 2W3X | none |
| DynE7g | M. chernisa | IPKS | TA | unconfirmed | 2010 | 2XFL | none |
| 2XEM | |||||||
| FcotT | M. tuberculosis | unknown | H3 | LC acyl-CoA | 2007 | 2PFC | dodecenoate |
| 2012 | 3B18 | dodecanoate | |||||
a
Abbreviations used: Bz, benzoyl; Bn, benzyl; Pa, phenacyl; Ac, acetyl; Hex, hexanoyl; CNAC, N-acetylaminopropyl; ONAC, N-acetylaminoethoxy; CoA, coenzyme A; PCP, peptidyl carrier protein; ACP, acyl carrier protein; LC, long-chain; MC, medium chain.
b
Terms defined as in Pidugu, et al.: D, dimer; TA, face-to-face dimer of dimers; TB, back-to-back dimer of dimers; H2 & H3, trimers of dimers; DdhB, DHD dimer corresponding to TB; Trdh, DHD trimer corresponding to H2.
c
An asterisk indicates a mutant.
d
Buffers and/or salt ions are not necessarily noted.
e
N-terminal domain only.
f
C-terminal domain only.
g
These enzymes may additionally perform decarboxylations.