Figure 1.
Immunofluorescence analysis of NS3 and VP5 co-localization with PI(4,5)P2 in BTV infected cells. HeLa cells either transfected with plasmid expressing PH-GFP (green)(B-C) or not transfected (E-F), were infected with BTV-1 and analyzed for localization with NS3 (A,E) and VP5 (B,F) 12hrs post-infection. Control consists of uninfected cells either expressing PH-GFP (C) or cells not expressing PH-GFP, but immulolabelled for PI(4,5)P2(D) (red). Area of co-localization shown by yellow has been magnified in the extreme right panel (E-F). In all the panels the nucleus (blue) was stained by Hoechst. The viral proteins (NS3 and VP5) were stained were either stained with TRITC (red) (A-B) or with FITC (green) (E-F). Small white arrows indicate the areas of co-localization shown by yellow. The scale has been included in the merged panel of each row.