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. 2013 Mar 5;10:73. doi: 10.1186/1743-422X-10-73

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Copyright ©2013 Bhattacharya and Roy; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Sequestration of virus proteins in Arf6/Q67L induced PI(4,5)P₂-enriched vesicles. Immunofluorescence microscopy of HeLa cells expressing HA-tagged Arf6/Q67L and PH-GFP followed by BTV-1 infection. The infected cells were analyzed 12hrs post-infection for localization of NS3 (B) and VP5 (C). Control consists of cells that have been co-transfected with PH-GFP and HA-tagged Arf6/Q67L, but not infected (A). While PH-GFP localization is shown in green, HA-tagged Arf6/Q67L was detected with Alexa Fluor (blue) and all the viral proteins with TRITC (red). White circles point to co-localized patches. The scale has been included in the merged panel of each row.