Table 4.
Results of qRT-PCR for the C. perfringens regulatory genes in the wild types and mutants
|
Gene ID and name |
Regulatory function |
qRT-PCR fold |
||
|---|---|---|---|---|
| |
|
|
change (mt/wt) |
|
| NCTR | ATCC13124 | |||
| CPE_1501 |
CPF_1752 (virR) |
DNA binding response regulator, VirR |
7.4 |
1.3 |
| CPE_1500 |
CPF_1751 (virS) |
sensor histidine kinase, VirS |
9.7 |
0.3 |
| CPE_0646 |
CPF_0627 (virX) |
conserved hypothetical protein |
2.2 |
−3.0 |
| CPE_0957 |
CPF_1204 (vrr) |
VR-RNA |
2.0 |
−158.5 |
| CPE_1701 |
CPF_1955 (codY) |
GTP-sensing transcriptional pleiotropic repressor CodY |
6.9 |
−1.8 |
| CPE_0073 |
CPF_0069 |
Transcription antiterminator |
1.5 |
−116.5 |
| CPE_0642 | CPF_0623 (RevR) | DNA binding response regulator | 2 | −2 |