Figure 4.

Methylation of CpG sites at, and upstream of, the transcription start site of the Slc2a4 gene. The promoter region and transcription start site of the Slc2a4 gene were targeted to establish if differential DNA methylation was occurring at specific CpG sites within these regions. The upper panel represents the position of CpG islands, target amplicons, and CpG sites within the target region, while the graph represents mean ± SEM for the percentage methylation at each site, as measured using Sequenom EpiTYPER. No significant differences were observed as a result of selenium and folate supplementation for any of the CpG sites measured.