Table 1.

Primers for MSP

Gene	Forward primer 5′-3′	Reverse primer 5′-3′	Tm,°C
GATA4-M	GTATAGTTTCGTAGTTTGCGTTTAGC	AACTCGCGACTCGAATCCCCG	62
GATA4-U	TTTGTATAGTTTTGTAGTTTGTGTTTAGT	CCCAACTCACAACTCAAATCCCCA	62
DcR1-M	TTACGCGTACGAATTTAGTTAAC	TCAACGACCGACCGAAACG	60
DcR1-U	GAATTTTTTTATGTGTATGAATTTAGTTAAT	CCATCAAACAACCAAAACA	60

M – methylated, U – unmethylated, Tm – melting temperature.