Table 2.
Details of oligonucleotide primers used for RT-LAMP assay
| Primer name | sequence(5’-3’) | Genome position |
|---|---|---|
| FIPa = F2 + F1c |
GTTGTGATCTGTTCAACGTGAATGA-gaattc-GGGTTTTACATTTCATAAACCAA |
F1c,510-534 F2,467-489 |
| BIPb = B2 + B1c |
GCTCATGATAACTTGATGGG- gatatc-GCACATGAGTAGTCGAATCC |
B1c,537-556 B2,597-616 |
| F3 |
TTGCAAAATAGAAGACAAAGAAC |
444-466 |
| B3 |
GTTGCGTATTAGCTGGCG |
625-642 |
| LoopF |
CTGAATAAGGGAAAATGTTTGGTTT |
483-507 |
| loopB | GATGTGGCTCAATGCGGG | 560-577 |
Genome position according to the bovine rotavirus complete genome sequence (GenBank accession no. K02254.1).
a FIP is composed of F2 and F1c, and they are linked by a sequences of EcoRIof -gaattc-.
b BIP is composed of B2 and B1c, and they are linked by a sequences of EcoRV of -gatatc-.