Table 3.
Comparison of real-time RT-PCR and RT-LAMP for the detection of BRV in clinical samples
| Location of samples | Number of Samples |
Number of positive samples for assay |
||
|---|---|---|---|---|
| Real-time RT-PCR | RT-LAMP | Sequencing | ||
| Nanning |
24 |
7 |
7 |
correct |
| Liuzhou |
15 |
3 |
3 |
correct |
| Fangcheng |
10 |
3 |
3 |
correct |
| Shangsi |
13 |
5 |
5 |
correct |
| Guilin |
20 |
10 |
10 |
correct |
| Hengxian |
16 |
1 |
1 |
correct |
| Total | 88 | 29 | 29 | correct |
a RT-LAMP -positive samples were all confirmed to be BRV by sequencing the RT-LAMP- products restriction analysis of VP6 genes.