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TNF-α and IL-10 production by infected MØ. Resting MØ and IFN-γ-activated MØ were infected with wild-type, ∆kstD, or ∆kstD-kstD strains for 2 hours and then cultured for 1 day. The amount of released TNF-α (A) and IL-10 (B) was assessed in culture supernatants using ELISA kits. Data are presented as means (pg/ml) ± SEMs (n = 5; *p ≤ 0.02, ∆kstD vs. wild-type or ∆kstD-kstD; Mann–Whitney U test). ops – bacteria opsonized, non-ops – bacteria non-opsonized.
