Table 2.

Overall mismatch rates in overlapping paired-end reads for raw read pairs, read pairs that have quality scores Q10, Q≥20, Q≥30 and Q≥35

Sample	Raw	Q10 (1×10-1)	Q20 (1×10-2)	Q30 (1×10-3)	Q35 (3×10-4)
BCV control	5.55×10-3	7.82×10-4	9.56×10-5	1.94×10-6	4.26×10-8
Rabies control	7.33×10-3	9.64×10-4	1.12×10-4	2.59×10-6	1.31×10-7
BCV	4.91×10-3	7.87×10-4	1.06×10-4	2.55×10-6	1.51×10-7
Fox1	8.92×10-3	1.01×10-3	1.73×10-4	8.82×10-6	6.92×10-7
Fox2	6.69×10-3	8.33×10-4	1.22×10-4	4.16×10-6	2.28×10-7
Average of 5 samples	6.68×10-3	8.75×10-4	1.22×10-4	4.01×10-6	2.49×10-7
Rabies control –repeat run	5.99×10-3	2.90×10-3	1.04×10-3	2.67×10-4	8.00×10-5

The rabies control plasmid was sequenced a second time using a separate protocol at a different service provider and listed here for comparison. Mismatch rates are ‘per base pair’ so that they can be compared directly across samples and platforms. Phred error rates for the Q scores are shown in the heading.