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Immunofluorescence staining of hAECs negative for DAZL, ZP3 and GDF9. (A) Freshly isolated hAECs; (B) hAECs cultured in medium containing SSS at passage 1 and at passage 3 (C). hAECs cultured in standard medium at passage 1 (D), at passage 3 (E) and at passage 5 (F). Blue color, DAPI nuclear staining of the corresponding cells.
