Table 1.
Primer sequences and the conditions of RT-PCR
| Primer | Sequence (5’ → 3’) | Product size (bp) | Annealing temperature (°C) | Cycles |
|---|---|---|---|---|
|
GAPDH |
FW- TGATGACATCAAGAAGGTGGTGAAG |
230 |
57 |
30 |
| |
REV -TCCTTGGAGGCCATGTGGGCCAT |
|
|
|
|
C-KIT |
FW- TTGTGCACGACGATGTCTGA |
296 |
57 |
30 |
| |
REV- AGTCCATACCTCCCTCTCTT |
|
|
|
|
DAZL |
FW- GAGTGGTCAAAGGAGCCAAA |
144 |
57 |
30 |
| |
REV- AGGAGCCACCTCCCTGAG |
|
|
|
|
VASA |
FW- TCTTCACAAGCTCCCAATCC |
165 |
57 |
30 |
| |
REV- TGAGAATACAAGGACAGGAGCT |
|
|
|
|
ZP3 |
FW- GCTTTGCCTTCTGACACCTC |
127 |
57 |
30 |
| |
REV- CAGACACAGGGTGGGAGG |
|
|
|
|
DMC1 |
FW- AGCAGCAAAGTTCCATGAAG |
300 |
56 |
30 |
| |
REV- TGAGCTCTCCTCTTCCCTTT |
|
|
|
|
SCP3 |
FWD-TGGAAAACACAACAAGATCA |
344 |
56 |
30 |
| |
REV-GCTATCTCTTGCTGCTGAGT |
|
|
|
|
STRA8 |
FW-CCTCAAAGTGGCAGGTTCTGAA |
126 |
56 |
30 |
| REV-TCCTCTAAGCTGCTTGCATGC |