Table 1.
Effects of gestational Se supply and nutritional plane on mammary gland weight, cellularity, cell proliferation, and vascularity when necropsied after parturition
| |
Se supply1 |
Nutritional plane2 |
P-value3 |
|||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Item | ASe | HSe | SEM4 | RES | CON | EXC | SEM5 | Se | Nut | Se×Nut |
| Final BW, kg |
54.5 |
54.9 |
1.4 |
46.3a |
54.0b |
63.8c |
1.7 |
0.86 |
<0.001 |
0.79 |
| Empty BW6, kg |
47.8 |
47.9 |
1.3 |
40.2a |
47.2b |
56.2c |
1.7 |
0.96 |
<0.001 |
0.87 |
| Mammary gland, g |
754.1 |
850.7 |
46.9 |
715.3a |
773.5ab |
918.4b |
59.6 |
0.15 |
0.05 |
0.55 |
| g/kg EBW7 |
15.9 |
18.2 |
1.1 |
18.0 |
16.7 |
16.4 |
1.4 |
0.14 |
0.69 |
0.33 |
| DNA, mg/g |
3.59 |
3.59 |
0.24 |
3.63 |
3.62 |
3.53 |
0.30 |
0.98 |
0.96 |
0.94 |
| DNA, g |
2.67 |
3.00 |
0.22 |
2.58 |
2.79 |
3.14 |
0.28 |
0.30 |
0.36 |
0.48 |
| RNA, mg/g |
5.49 |
5.42 |
0.38 |
5.60ab |
6.21a |
4.56b |
0.48 |
0.90 |
0.04 |
0.90 |
| RNA, g |
3.89 |
4.55 |
0.30 |
3.92 |
4.57 |
4.17 |
0.39 |
0.13 |
0.46 |
0.78 |
| RNA:DNA |
1.57 |
1.52 |
0.09 |
1.57ab |
1.71a |
1.36b |
0.11 |
0.67 |
0.07 |
0.41 |
| Protein, mg/g |
29.37 |
25.64 |
2.64 |
25.02 |
27.72 |
29.79 |
3.36 |
0.32 |
0.59 |
0.25 |
| Protein, g |
22.75 |
21.54 |
2.27 |
18.16a |
21.16ab |
27.12b |
2.89 |
0.71 |
0.09 |
0.10 |
| Protein:DNA |
8.80 |
7.98 |
1.03 |
7.86 |
7.76 |
9.54 |
1.32 |
0.57 |
0.53 |
0.51 |
| Proliferation, % |
3.38 |
2.14 |
0.40 |
1.63 |
2.96 |
3.69 |
0.51 |
0.02 |
0.03 |
0.07 |
| ASe |
-- |
-- |
-- |
1.82y |
3.06y |
5.27z |
0.75 |
|
|
|
| HSe |
-- |
-- |
-- |
1.44y |
2.86y |
2.11y |
0.68 |
|
|
|
| Vascular area, % |
48.74 |
51.27 |
1.08 |
50.78 |
49.47 |
49.77 |
1.35 |
0.09 |
0.76 |
0.33 |
| Alveoli per area7 | 0.57 | 0.53 | 0.02 | 0.52 | 0.55 | 0.59 | 0.02 | 0.23 | 0.10 | 0.46 |
a,b,c Within an item, main effect means differ (P ≤ 0.05).
y,z Within an item, interactive means differ (P ≤ 0.02).
1Ewes fed 11.5 μg/kg BW Se (ASe) or 77.0 μg/kg BW Se (HSe) during gestation.
2Ewes fed 60% (RES), 100% (CON), or 140% (EXC) of nutrient requirements from d 40 of gestation to term.
3Probabilities of difference for Se supply (Se), nutritional plane (Nut), and the interaction (Se×Nut).
4Standard error means for ASe n = 19 and HSe n = 19.
5Standard error means for RES n = 12, CON n = 14, and EXC n = 12.
6Empty BW (EBW) = Final BW – digesta weight.
7Alveoli per area = (number of alveoli/total picture area) ×1,000.