Table 2.
Effects of gestational Se supply and nutritional plane on mammary gland weight, cellularity, cell proliferation, and vascularity when necropsied after d 20 of lactation
| |
Se supply1 |
Nutritional plane2 |
P-value3 |
|||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Item | ASe | HSe | SEM4 | RES | CON | EXC | SEM5 | Se | Nut | Se×Nut |
| Final BW, kg |
55.9 |
57.3 |
1.3 |
48.5a |
56.7b |
64.6c |
1.6 |
0.46 |
<0.001 |
0.56 |
| Empty BW6, kg |
45.9 |
47.73 |
1.25 |
39.1a |
46.9b |
54.4c |
1.6 |
0.29 |
<0.001 |
0.31 |
| Mammary gland, g |
577.7 |
634.4 |
31.7 |
541.8 |
628.1 |
648.4 |
38.8 |
0.21 |
0.13 |
0.74 |
| g/kg EBW7 |
12.6 |
13.5 |
0.8 |
14.0 |
13.4 |
11.7 |
0.9 |
0.42 |
0.19 |
0.78 |
| DNA, mg/g |
3.43 |
3.26 |
0.12 |
3.15 |
3.48 |
3.40 |
0.14 |
0.30 |
0.26 |
0.89 |
| DNA, g |
1.98 |
2.10 |
0.15 |
1.71a |
2.23b |
2.18b |
0.18 |
0.54 |
0.09 |
0.96 |
| RNA, mg/g |
8.50 |
8.10 |
0.39 |
7.46 |
8.76 |
8.68 |
0.48 |
0.48 |
0.11 |
0.37 |
| RNA, g |
5.00 |
5.22 |
0.43 |
4.14a |
5.59b |
5.60b |
0.52 |
0.71 |
0.09 |
0.69 |
| RNA:DNA |
2.49 |
2.49 |
0.09 |
2.39 |
2.52 |
2.56 |
0.11 |
0.99 |
0.53 |
0.13 |
| Protein, mg/g |
27.71 |
30.83 |
2.62 |
28.19 |
30.40 |
29.23 |
3.21 |
0.41 |
0.89 |
0.48 |
| Protein, g |
16.20 |
19.77 |
2.05 |
15.37 |
19.33 |
19.25 |
2.51 |
0.22 |
0.45 |
0.74 |
| Protein:DNA |
8.26 |
9.55 |
0.87 |
9.18 |
8.93 |
8.60 |
1.07 |
0.30 |
0.93 |
0.50 |
| Proliferation, % |
9.81 |
11.15 |
1.34 |
10.55 |
11.11 |
9.78 |
1.64 |
0.85 |
0.48 |
0.62 |
| Vascular area, % |
45.37 |
48.59 |
1.36 |
48.20 |
46.75 |
46.00 |
1.69 |
0.10 |
0.64 |
0.08 |
| ASe |
-- |
-- |
-- |
48.01y |
42.07z |
46.03yz |
2.48 |
|
|
|
| HSe |
-- |
-- |
-- |
48.38y |
51.42y |
45.96yz |
2.29 |
|
|
|
| Alveoli per area7 | 0.54 | 0.52 | 0.03 | 0.45a | 0.55b | 0.59b | 0.03 | 0.45 | 0.02 | 0.33 |
a,b,c Within an item, main effect means differ (P ≤ 0.07).
y,z Within an item, interactive means differ (P ≤ 0.09).
1Ewes fed 11.5 μg/kg BW Se (ASe) or 77.0 μg/kg BW Se (HSe) during gestation.
2Ewes fed 60% (RES), 100% (CON), or 140% (EXC) of nutrient requirements from d 40 of gestation to term.
3Probabilities of difference for Se supply (Se), nutritional plane (Nut), and the interaction (Se×Nut).
4Standard error means for ASe n = 21 and HSe n = 21.
5Standard error means for RES n = 14, CON n = 14, and EXC n = 14.
6Empty BW (EBW) = Final BW – digesta weight.
7Alveoli per area = (number of alveoli/total picture area) ×1,000.