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. Author manuscript; available in PMC: 2013 Mar 16.
Published in final edited form as: J Neurochem. 2007 Dec 10;105(3):763–772. doi: 10.1111/j.1471-4159.2007.05178.x

Figure 4.

Figure 4

Two-dimensional Oxyblot analysis of EAE spinal cord proteins. Spinal cord homogenates from EAE animals were derivatized with 2, 4-dinitrophenylhydrazine. Proteins were separated by 2-D-gel electrophoresis and blotted to PVDF membranes. Membranes were immunostained with anti-DNP, then stripped and re-probed with antibodies against the major cytoskeletal proteins. Cytoskeletal proteins on anti-DNP blots were identified by spot matching using BioRad PD-Quest software. The major carbonylated targets in EAE include β-actin, GFAP and the neurofilament proteins.