Table 2.
DNA concentration ng/μL, presented as median (maximum – minimum) measured using three different measurement methods
| Extraction method | UV spectroscopy | qPCR | Fluorometry |
|---|---|---|---|
| Reference |
524.4 (1502.2 − <LOQ*) |
144.2 (487.7 − 0.02) |
84.2 (460.0 − <LOQ) |
| PrecipB |
61.1 (178.3 − 3.8) |
101.5 (149.4 − 0.00) |
51.8 (178.4 − 3.8) |
| ChemLysis |
16.5 (110.2 − <LOQ) |
2.1 (47.7 − 0.19) |
2.2 (55.2 − <LOQ) |
| PrecipG |
14.9 (44.4 − <LOQ) |
8.4 (94.5 − 0.17) |
6.2 (29.6 − <LOQ) |
| PrecipS |
4.0 (12.0 − <LOQ) |
1.5 (6.1 − 0.01) |
1.4 (9.5 − <LOQ) |
| MagBeads | 4.3 (7.6 − <LOQ) | 0.2 (1.3 − <LOQ) | 0.4 (2.4 − <LOQ) |
* below the method limit of quantification (LOQ).