Table 1.
Anti-plasmodial activity (IC50) and cytotoxicity (CC50) of HSCCC fractions of Annickia kummeriae methanolic leaf extract
| Fraction | Wt (mg) | P. falciparum K1 IC50 (μg/ml) | Cytotoxicity CC50 (μg/ml) | SI | IC50 fr. IC50 CQ | IC50 fr. IC50 Art | IC50 fr. IC50 Pdx |
|---|---|---|---|---|---|---|---|
| AKLM |
15,000 |
0.12 ± 0.01 |
30.0 ± 0.8 |
250 |
1.9 |
60 |
3,333 |
| AKLM1 |
562 |
5.0 ± 0.31 |
>90.0 |
>18 |
79.4 |
2500 |
>10,000 |
| AKLM2 |
2,999 |
0.87 ± 0.1 |
20.0 ± 3.3 |
23 |
13.8 |
435 |
2,222 |
| AKLM3 |
1,534 |
3.01 ± 0.81 |
78.0 ± 5.4 |
26 |
47.8 |
1,505 |
8,667 |
| AKLM 4 |
157 |
1.34 ± 0.33 |
76.0 ± 1.44 |
57 |
21.3 |
670 |
8,444 |
| AKLM 5 |
249 |
3.6 ± 0.2 |
>90.0 |
>25 |
57.1 |
1,800 |
>10,000 |
| AKLM 6 |
427 |
0.45 ± 0.15 |
59.0 ± 1.5 |
131 |
7.1 |
225 |
6,556 |
| AKLM 7 |
165 |
0.11 ± 0.02 |
>90.0 |
>818 |
1.7 |
55 |
>10,000 |
| AKLM 8 |
221 |
0.09 ± 0.04 |
>90.0 |
>1,000 |
1.4 |
45 |
>10,000 |
| AKLM 9 |
680 |
0.06 ± 0.02 |
>90.0 |
>1,500 |
1.0 |
30 |
>10,000 |
| AKLM 10 |
1,295 |
0.05 ± 0.02 |
>90.0 |
>1,800 |
0.8 |
25 |
>10,000 |
| AKLM 11 |
1,679 |
0.05 ± 0.01 |
>90.0 |
>1,800 |
0.8 |
25 |
>10,000 |
| AKLM 12 |
1,056 |
0.06 ± 0.03 |
>90.0 |
>1,500 |
1.0 |
30 |
>10,000 |
| AKLM 13 |
878 |
0.62 ± 0.4 |
>90.0 |
>145 |
9.8 |
310 |
>10,000 |
| AKLM 14 |
948 |
1.0 ± 0.22 |
>90.0 |
>90 |
15.9 |
500 |
>10,000 |
| AKLM 15 |
1,232 |
0.13 ± 0.02 |
>90.0 |
>692 |
2.1 |
65 |
>10,000 |
| AKLM 16 |
416 |
0.09 ± 0.03 |
84.0 ± 3.91 |
933 |
1.4 |
45 |
9,333 |
| AKLM 17 | 498 | 1.67 ± 0.43 | >90.0 | >54 | 26.5 | 835 | >10,000 |
Fr. – HSCCC fraction of Annickia kummeriae leaves methanolic extract, P. falciparum K1 used for anti-plasmodial assays, rat myoblast L-6 cells used for cytotoxicity assays, CQ chloroquine (IC50 0.063 ± 0.034 μg/ml), Art artemisinin (IC50 0.002 ± 0.00001 μg/ml), Pdx podophyllotoxin (IC50 0.009 ± 0.003 μg/ml).