Table 2.
Anti-plasmodial activity (IC50) and cytotoxicity (CC50) data of sub-fractions of fraction 2 of Annickia kummeriae methanolic leaf extract
| Fraction | Weight (mg) | P. falciparum K1 IC50 (μg/ml) | Cytotoxicity CC50 (μg/ml) | SI | IC50 fr. IC50CQ | IC50 fr. IC50 Art | CC50 fr. CC50 Pdx |
|---|---|---|---|---|---|---|---|
| AKLM 2 |
2,500 |
0.90 ± 0.11 |
21.0 ± 3.87 |
23.3 |
14 |
450 |
3,500 |
| AKLM 2.1 |
15.3 |
5.0 ± 1.31 |
>90 |
18.0 |
78 |
2,500 |
>15,000 |
| AKLM 2.2 |
26.9 |
1.09 ± 0.26 |
79.1 ± 7.60 |
72.6 |
17 |
545 |
13,183 |
| AKLM 2.3 |
57.6 |
4.11 ± 0.29 |
53.1 ± 9.20 |
12.9 |
64 |
2,055 |
8,850 |
| AKLM 2.4 |
98.7 |
1.16 ± 0.18 |
13.8 ± 1.80 |
11.9 |
18 |
580 |
2,300 |
| AKLM 2.5 |
126.1 |
3.63 ± 0.04 |
24.6 ± 3.30 |
6.8 |
57 |
1,815 |
4,100 |
| AKLM 2.6 |
115.7 |
1.23 ± 0.31 |
39.5 ± 2.20 |
32.1 |
19 |
615 |
6,583 |
| AKLM 2.7 |
239.0 |
3.41 ± 0.48 |
85.4 ± 4.60 |
25.0 |
53 |
1,705 |
14,233 |
| AKLM 2.8 |
478.3 |
5.0 ± 0.53 |
>90 |
18.0 |
78 |
2,500 |
>15,000 |
| AKLM 2.9 |
351.8 |
2.40 ± 0.57 |
56.7 ± 8.00 |
23.6 |
38 |
1,200 |
9,450 |
| AKLM 2.10 |
301.9 |
0.89 ± 0.20 |
35.3 ± 5.31 |
39.7 |
14 |
445 |
5,883 |
| AKLM 2.11 | 672.8 | 0.64 ± 0.34 | 44.7 ± 4.45 | 69.8 | 10 | 320 | 7,450 |
HSCCC High speed counter current chromatography; AKLM Annickia kummeriae leaf methanol extract, P. falciparum K1 used for anti-plasmodial assays, rat myoblast L-6 cells used for cytotoxicity assays, CQ chloroquine (IC50 0.063 ± 0.034 μg/ml), Art - artemisinin (IC50 0.002 ± 0.00001 μg/ml), Pdx - podophyllotoxin (IC50 0.009 ± 0.003 μg/ml).