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Primers used to amplify HKT1;5 from rice
| Primer | Sequence (5′ – 3′) | Tm (°C) | Size (bp) |
|---|---|---|---|
| Tile8For |
GTCGCCTCCCTCCAGCTAATGTACTGTC |
78.7 |
3102 |
| Tile8Rev |
GGCCTCCAACAAACTGAAAGCGTCAAT |
79.6 |
|
| Tile9For |
GGCGGTGGGTGGTGCTTGGGTAGAGATA |
83.9 |
1806 |
| Tile9Rev |
GATGACAAGAGCGGCCGACAGTACATTA |
78.8 |
|
| Tile10For |
CTACACTGAATTATACTGCGTGAAC |
65.5 |
1390 |
| Tile10Rev | TAGAGCTCGACCAGATCCTGATATAGAC | 71.1 |
Primers used to amplify HKT1;5 from O. sativa and O. glaberrima.
