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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: Pancreas. 2013 Apr;42(3):467–474. doi: 10.1097/MPA.0b013e318264d0f8

Fig.4. Effects of EP2 and EP4 silencing on PGE2 mediated HPSC functions.

Fig.4

HPSC were transiently transfected with siControl, siEP2 or siEP4. After 48 hours, cell migration and invasion were assessed by counting the number of stained cells that penetrated the migration membrane after 24h following PGE2 (100nM) stimulation. (A) membrane alone (right, stained cells; left migration numbers p<0.05 versus control) (B) matrigel coated membrane (right, stained cells; left migration numbers p<0.05 versus control) (C) RT-PCR shows the effects of PGE2 mediated stimulation of COL1A1, MMP-2 and 9 after the silencing of EP4 receptor. β-actin served as control. (D) Q-RT-PCR shows the effects of PGE2 mediated stimulation of COL1A1, MMP-2 and 9 and decrease after the silencing of EP4 receptor. β-actin served as control.