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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: Pancreas. 2013 Apr;42(3):467–474. doi: 10.1097/MPA.0b013e318264d0f8

Fig.5. Effects of specific EP receptor antagonists on PGE2 mediated HPSC functions.

Fig.5

HPSC were plated and allowed to settle overnight. Serum containing media was re-plated with serum free media for 24 hours before addition of EP1, EP2 or EP4 antagonists at a dose of 10μM. 1 hour after the antagonist addition, cell migration and invasion were assessed by counting the number of DAPI stained cells that penetrated the migration membrane after 24 h following PGE2 (100nM) stimulation. (A) membrane alone (right, stained cells; left migration numbers p<0.05 versus control) (B) matrigel coated membrane (right, stained cells; left migration numbers p<0.05 versus control). Addition of EP4 antagonist showed a significant reduction of PGE2 mediated HPSC migration and invasion. (C) RT-PCR showing the reduction in collagen 1A1 expression on treatment with EP4 antagonist (10μM) as compared to increase on treatment with PGE2 (100nM) alone.