|
EP1: (5′ atc gct tcg gcc tcc acc ttc ttt 3′) |
(3′ gcc agc gcc acc aac acc a 5′) |
|
EP2: (5′ctc gct gcc gct gct gga cta tgg3′) |
(3′gca ggc gag cac cga gac aat gag 5′) |
|
EP3: (5′ ggc gct ggc gat gaa caa cga g 3′) |
(3′ ggc gct gga gat gaa caa cga g 5′) |
|
EP4: (5′ ccg ccc cca ggt agc cag gag 3′) |
(3′tgc ggg agg aca gcg ttc agg t 5′) |
|
MMP-1: (5′ att cta ctg ata tcg ggg ctt tga3′) |
(3′ tgt cct tgg ggt atc cgt gtg tag5′) |
|
MMP-2: (5′ ctt ctt gtc gcg gtc gta gtc ctc3′) |
(3′ tgg cga tgg ata ccc cct tga5′) |
|
MMP-7: (5′aaa ctc ccc gcg tca tag aaa taa t3′) |
(3′ tga gtt gca gca tac agg aag tt 5′) |
|
MMP-9: (5′ gcg ctg ggc tta gat cat tcc tca 3′) |
(3′ gca gcg cgg gcc att gtc 5′) |
|
MMP-11: (5′ctg gcg ggc gct ggg aga aga c3′) |
(3′cag ggc tgg cca tat agg tgt tga5′) |
|
TIMP-1: (5′ cgt cat cag ggg cca agt tcg tg 3′) |
(3′ gag gca ggc agg caa ggt gac 5′) |
|
TIMP-2: (5′ ctg gcg ggc gct ggg aga agac3′) |
(3′cag ggc tgg cca tat agg tgt tga3′) |
|
Fibronectin: (5′ccg cca cgt gcc agg att acc3′) |
(5′agg ggc tcg ctc ttc tga tta ttc 3′) |
|
Collagen1A1: (5′tgt cca ccg agg ctc cca gaa c3′) |
(5′ ccc agg ctc cgg tgt gac tcg tg 3′) |
|
Vimentin: (5′ ggt ccg tgt cct cgt cct cct ac 3′) |
(3′ cgc ggg ctt tgt cgt tgg tta 5′) |
|
Elastin: (5′gga ccc ctg act cac gac ctc 3′) |
(5′ act tgg ccg ctc ccc tct tgt ttc 3′) |
|
HSPG2: (5′ ccg cca ggg cag gtc a 3′) |
(3′ ggt ggg cag cgg tag gaa gga gta 5′) |
|
COX-2: (5′ ggt ctg gtg cct ggt ctg atg atg 3′) |
(5′ gtc ctt tca agg aga atg gtg c 3′) |
|
β-actin: (5′ atg ata tcg ccg cgc tcg tcg tc 3′) |
(5′ cgc tcg gcc gtg gtg gtg aa 3′) |
| Q-RT-PCR Primers: |
| EP4: |
Hs00168761 |
| MMP2: |
Hs00234422 |
| MMP9: |
Hs00234579 |
| COL1A1: |
Hs00164004 |
|
β-actin: |
4352935E |
