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. Author manuscript; available in PMC: 2014 Mar 15.
Published in final edited form as: ACS Chem Biol. 2013 Jan 7;8(3):636–642. doi: 10.1021/cb300396j

Figure 6.

Figure 6

Fluorescence micrographs of unrestrained C. elegans during the DMP after co-feeding on a mixture of the green fluorescent probe Oregon Green dextran (25 μM) and either the red fluorescent probes RhB amide (10 μM, panel A) or KR35 (10 μM, panel B) for 2 h. Time-dependent images during the DMP extracted from video microscopy are shown. Fluorescence is rendered as a spectrum heat-map, with red representing the most intense fluorescence and black the least intense fluorescence. The head of the animal is on the left side of each image. Independent imaging of these probes confirmed spectral orthogonality with the filter sets employed. A major shift of fluorescence from the posterior intestine to the anterior-most intestine during the DMP was only observed with the acid activated KR35 fluorophore (panel B, t = 6.8 s). Scale bar = 100 microns.