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Summary of purification of endoglucanase fromBacillussp MTCC 10046
| Sample | Volume (ml) | Total protein | Protein conc. (mg/ml) | Total Activity (units) | Yield (%) | Specific activity (unit/mg) | Purification (fold) |
|---|---|---|---|---|---|---|---|
| Crude | 70 | 14.2 mg | 0.202 | 113.6 | 100 | 8.0 | 1 |
| 40-80% dialyzed fraction | 15 | 1.48 mg | 0.098 | 68.37 | 60.2 | 46.2 | 5.8 |
| DEAE column E5 | 6 | 0.66 mg | 0.11 | 44.9 | 39.5 | 68.1 | 8.5 |
Unit = release of reducing sugar in μmoles/ml/min.
