Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2014 Feb 15.

Published in final edited form as: Arch Biochem Biophys. 2013 Jan 25;530(2):101–107. doi: 10.1016/j.abb.2013.01.001

Fig. 7 — NMR spectra of (A) glycocholic acid and (B) synthetic glyco-7-oxo-deoxycholic acid (in CDCl₃, 600 MHz). Some of the key downfield peaks are identified on the spectra. (C) Co-chromatography of the enzymatic oxidation product with synthetic glyco-7-oxo-deoxycholic acid, monitoring relative abundance at m/z 464; (D) mass spectrum of the enzymatic oxidation product, showing the characteristic ammonium ion [M+NH₄]⁺ (m/z 481) in addition to [M+H]⁺ (m/z 464). See text for HRMS.

Fig. 7 — NMR spectra of (A) glycocholic acid and (B) synthetic glyco-7-oxo-deoxycholic acid (in CDCl₃, 600 MHz). Some of the key downfield peaks are identified on the spectra. (C) Co-chromatography of the enzymatic oxidation product with synthetic glyco-7-oxo-deoxycholic acid, monitoring relative abundance at m/z 464; (D) mass spectrum of the enzymatic oxidation product, showing the characteristic ammonium ion [M+NH₄]⁺ (m/z 481) in addition to [M+H]⁺ (m/z 464). See text for HRMS.