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Plasmids used or constructed in this work
| Name | Genotype |
|---|---|
| pTL1001 | E. coli-Streptomyces shuttle vector, temperature sensitive, thiostrepton resistant [15] |
| pCR@2.1 | Clone vector, Invitrogen |
| pTZ3099L | pCR@2.1 derivative |
| pTZ3099R | pCR@2.1 derivative |
| pTZ3099 | pTL1001 derivative, gene replacement of sco3099 |
| pTGV2 | E. coli-Streptomyces shuttle vector, thiostrepton resistant [14] |
| pTLV2 | Plasmid with insertion of 450 bp ermEp promotor |
| pTZ3099com | pTGV2 derivative, for complementation of sco3099 |
