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. Author manuscript; available in PMC: 2013 Sep 14.
Published in final edited form as: Nature. 2013 Feb 24;495(7440):231–235. doi: 10.1038/nature11885

Figure 2. CXCL12 produced by endothelial cells promotes HSC maintenance.

Figure 2

a, Bone marrow and spleen cellularity (n=8–9) and (b) HSC frequency in Tie2-cre; Cxcl12fl/fl mice versus littermate controls (n=10–11). c, 3×105 donor bone marrow cells from Tie2-cre; Cxcl12fl/fl mice gave significantly lower levels of donor myeloid, B, and T cell reconstitution in irradiated mice (three experiments with a total of 12–14 recipients per genotype). d–f, Tie2-cre; Cxcl12fl/fl mice had normal frequencies of MPPs, LMPPs, CMPs, MEPs, GMPs (d), CLPs (e), and committed B lineage progenitors in bone marrow (f) (n=3–4). g–i, Tie2-cre; Cxcl12fl/fl mice had normal frequencies of myeloerythroid colony-forming progenitors in bone marrow (g), spleen (h), and blood (i) (n=3–6). Δ, recombined Cxcl12fl allele; con, negative control mice with +/+ or fl/+ or fl/fl Cxcl12 genotypes (without cre). Data are mean±s.d. (*P<0.05, **P<0.01, ***P<0.001, #=0.057).