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. Author manuscript; available in PMC: 2013 Sep 14.
Published in final edited form as: Nature. 2013 Feb 24;495(7440):231–235. doi: 10.1038/nature11885

Figure 3. CXCL12 produced by Lepr-expressing perivascular stromal cells retains HSCs and colony-forming progenitors in the bone marrow.

Figure 3

a, b, Cellularity (a, n=6) and HSC frequency (b, n=6–7) in the bone marrow and spleen of Lepr-cre; Cxcl12fl/− mice and littermate controls. c, 3×105 bone marrow cells from Lepr-cre; Cxcl12fl/− mice gave normal levels of donor myeloid, B, and T cell reconstitution in irradiated mice (three experiments with a total of 15 recipient mice per genotype). d–f, Lepr-cre; Cxcl12fl/− mice had normal frequencies of MPPs, LMPPs, CMPs, MEPs, GMPs (d), CLPs (e), and committed B lineage progenitors in bone marrow (f) (n=3). g–i, Lepr-cre; Cxcl12fl/− mice had normal frequencies of myeloerythroid colony-forming progenitors in bone marrow (g) but significantly increased frequencies in spleen (h), and blood (i) (n=3–5). j, 6×105 mononucleated blood cells from Lepr-cre; Cxcl12fl/− mice gave long-term multilineage reconstitution in irradiated mice, while blood cells from littermate controls did not. Δ, recombined Cxcl12fl allele; -, germline deleted Cxcl12 allele or Cxcl12DsRed allele; con, control mice. Data represent mean±s.d. (*P<0.05, **P<0.01, ***P<0.001).