Figure 5.
Real-time QPCR allows potential hits to be stratified based on selectivity and specificity. Real-time QPCR was done using viral (vGPCR) and cellular (U6) primer sets. For uninfected BJAB (A) and naturally infected BCBL-1 cells (B), cell viability of extract-treated cells was normalized to the DMSO control using the equation: 1.9−ΔCT(U6). C, cellular and viral DNA in extract-treated KSHV-BJAB cells were first normalized to DMSO-treated KSHV-BJAB cells. Then, the selectivity index was calculated as the ratio of changes in viral DNA to changes in cellular DNA.