Figure 6.
Influence of 5,7-dihydroxyflavone on the expression of Bcl-2, Bax, Bcl-XL, and Mcl-1. (a) HepG2 cells were treated with the indicated concentrations of 5,7-dihydroxyflavone for 24 h. Western blotting was performed to determine the protein levels of Bcl-2, Bax, Bcl-XL, and Mcl-1. GADPH was used as loading control. (b)–(d) the relative densities of these proteins were determined following densitometric measurements of the specific protein bands and normalization against the value of GAPDH. The asterisk indicates a significant difference (*P < 0.05, **P < 0.01) compared with control. Data are mean ± SD.