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. 2013 Feb 28;2013:434709. doi: 10.1155/2013/434709

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Copyright © 2013 Zhenzhen Zhang et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Influence of 5,7-dihydroxyflavone on the activation of Akt, STAT3, and MAPK. (a) HepG2 cells were treated with the indicated concentrations of 5,7-dihydroxyflavone for 24 h. Western blotting was performed to determine the phosphorylation levels of Akt, STAT3, and mitogen-activated protein kinase (MAPK). GADPH was used as loading control. (b, c), the relative densities of phospho-Akt and phosphor-STAT3 were determined following densitometric measurements of the specific protein bands and normalization against the value of total Akt and STAT3, respectively. The asterisk indicates a significant difference (**P < 0.01) compared with control. Data are mean ± SD.