Table 1.
Biochemical properties of the selected peptide clones (from http://www.expasy.ch/tools/protparam.html).
| Clone | Sequence | T1/2 (hours) | pI |
|---|---|---|---|
| C2-R3 | C-GSKTQAP-C | 30 | 8.06 |
| C18-R3 | C-GLPWLST-C | 30 | 5.51 |
| C22-R3 | C-STPHNLG-C | 1.9 | 6.72 |
| C25-R3 | C-LATGNQI-C | 5.5 | 5.51 |
| C30-R3 | C-PATLTSL-C | 20 | 5.51 |
| C33-R3 | C-HGAPNRL-C | 3.5 | 8.08 |
| C41-R3 | C-RPPIGAF-C | 1 | 8.07 |
| C42-R3 | C-TSQSQHM-C | 7.2 | 6.72 |
| C45-R3 | C-GPAVYMK-C | 30 | 8.05 |
| C46-R3 | C-QGDLPGY-C | 0.8 | 3.80 |
| C32-R4 | C-SPHTTIA-C | 1.9 | 6.72 |
| C33-R4 | C-EPFAGRS-C | 1 | 5.99 |
| C35-R4 | C-TSPLPGT-C | 7.2 | 5.51 |
| C46-R4 | C-SYEAHQT-C | 1.9 | 5.24 |
| C47-R4 | C-NNPLKSL-C | 1.4 | 8.06 |
T1/2: half-life theoretically estimated in mammalian reticulocytes in vitro and based on the N-end rule [20].
pI: isoelectric point which is the pH at which a particular molecule or surface carries no net electrical charge.