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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: J Invest Dermatol. 2012 Dec 6;133(4):988–998. doi: 10.1038/jid.2012.437

Figure 1. TLR2 agonists enhance TJ function in PHK.

Figure 1

TEER was measured daily until day 8 in PHK stimulated with (a) S. aureus- derived peptidoglycan (PGN, n= 3–7), (b) S. aureus-derived lipoteichoic acid (LTA, n= 3), (c) Pam3CSK4, a TLR1/2 ligand (n= 3–6), (d) Malp-2, a TLR2/6 ligand (n= 5), (e) Lipopolysaccharide (LPS), a TLR4 ligand (n=3) and (f) poly I:C, a TLR3 ligand (n= 3–9). Data are presented as the mean ± SEM by analyzing the area under the TEER curve normalized to the mean values for the control group (media alone). (g) PHK grown on Transwell™ inserts were stimulated with the indicated concentrations of S. aureus-derived PGN, Pam3CSK4 and Malp-2 for 48 h and paracellular permeability measured and compared to media alone. Data are expressed as a fold of media alone group. n=3; *P <0.05; **P <0.01; ***P < .001; ns: not significant.