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. Author manuscript; available in PMC: 2013 Nov 1.

Published in final edited form as: Kidney Int. 2012 Dec 12;83(5):845–854. doi: 10.1038/ki.2012.393

Fig. 6 — A. Representative morphology of MCs before and 30 min after 1 μM Ang II stimulation. Cells were treated with DMSO (vehicle) or bardoxolone methyl (BARD) at 500 nM for ~16 h at 37°C. B. Dose dependent effect of bardoxolone methyl on Ang II-induced MC contraction. Cells were treated with DMSO or various concentrations of bardoxolone methyl for ~16 h at 37°C. UT, untreated; * P<0.05, compared with DMSO group. The numbers in bars indicate the number of cells analyzed. C. Time course effect of bardoxolone methyl on Ang II-induced MC contraction. MCs were treated with either DMSO or bardoxolone methyl at 500 nM for different time periods at 37°C. * P<0.05, compared with both 0 and 3 min groups. The numbers inside bars indicate the number of cells analyzed. D: Percent inhibition was calculated by normalizing responses in bardoxolone methyl-treated cells to the response in DMSO-treated cells. The data was fit to a sigmoidal curve with 3 parameters and IC₅₀ was calculated as a function of the fitting curve.

Fig. 6 — A. Representative morphology of MCs before and 30 min after 1 μM Ang II stimulation. Cells were treated with DMSO (vehicle) or bardoxolone methyl (BARD) at 500 nM for ~16 h at 37°C. B. Dose dependent effect of bardoxolone methyl on Ang II-induced MC contraction. Cells were treated with DMSO or various concentrations of bardoxolone methyl for ~16 h at 37°C. UT, untreated; * P<0.05, compared with DMSO group. The numbers in bars indicate the number of cells analyzed. C. Time course effect of bardoxolone methyl on Ang II-induced MC contraction. MCs were treated with either DMSO or bardoxolone methyl at 500 nM for different time periods at 37°C. * P<0.05, compared with both 0 and 3 min groups. The numbers inside bars indicate the number of cells analyzed. D: Percent inhibition was calculated by normalizing responses in bardoxolone methyl-treated cells to the response in DMSO-treated cells. The data was fit to a sigmoidal curve with 3 parameters and IC₅₀ was calculated as a function of the fitting curve.