Table 1.

Experimental data sets used in this work and models derived from them

Data set	Description	Receptor-binding models derived from data
SPOT substitution arrays	170 one-residue variants of Bim BH3 (binding to all 5 receptors), 222 one-residue variants of Bad BH3 (binding Bcl-xL) and Noxa BH3 (binding Mcl-1)	Bcl-xL: PSSMSPOTX_Bim, PSSMSPOTX_Bad Mcl-1: PSSMSPOTM_Bim, PSSMSPOTM_Noxa Bcl-2: PSSMSPOT2_Bim, Bcl-w: PSSMSPOTW_Bim Bfl-1: PSSMSPOTF_Bim
SPOT library arrays	359 sequences including 1–5 mutations in Bim BH3 (binding to Mcl-1 and Bcl-xL)	N/A
Specific tight binders	Conventional sequencing of individual yeast clones assessed for binding to Bcl-xL and Mcl-1: 40 Bcl-xL and 33 Mcl-1-specific sequences	N/A
Likely binders	Illumina deep sequencing of yeast clones screened for high-affinity binding to receptors	Bcl-xL: PSSMDEEPX_AFFIN, Mcl-1: PSSMDEEPM_AFFIN Bcl-2: PSSMDEEP2_AFFIN, Bcl-w: PSSMDEEPW_AFFIN Bfl-1: PSSMDEEPF_AFFIN
Likely specific binders	Illumina deep sequencing of yeast clones screened for Mcl-1 versus Bcl-xL specific binding and vice versa	Bcl-xL: PSSMDEEPX_SPEC, Mcl-1: PSSMDEEPM_SPEC

Mcl-1 and Bcl-x_L substitution and library SPOT array data and yeast screening experiments are from Dutta et al.¹⁴

N/A - Not applicable.