Fig. 3.
Regulation of genes downstream of Cry2, Pdp1iso1, and JH/Met in the gut. (A) Reproductive (i.e., LD) and diapause (i.e., SD) females were injected with dsRNAs and, after 4 d, treated with methoprene as indicated below the columns. Four days later, mRNA levels were measured in their guts for Pdp1iso1, lip, def, and est genes, whose expression characterizes the reproductive state (Upper), and for cry2, tf, and sod genes, which are active under diapause (Lower). (B) Expression of the reproduction and diapause downstream genes in isolated guts. Guts were dissected from control or from diapause (i.e., SD) females 2 d after dsRNA injection, and were cultured for 2 d with or without methoprene as indicated. Alternatively, isolated guts were exposed to cry2 dsRNA for 2 d in culture (asterisk). All data have been normalized to rp49 expression and are shown relative to the levels of reproduction downstream genes in untreated LD females (Upper) and to the levels of diapause downstream genes in untreated SD females (Lower), respectively, that were set to 100%. Statistical significance of the differences is shown in Table S1.