Fig. 3.

Requirement of BCL2A1 for melanoma growth. (A) Colony formation assay of cell lines 72 h after transfection with siRNA targeting BCL2A1. Results are normalized to control siRNA. Data represent an average of at least three independent experiments with SE. ***P < 0.001 compared with siControl. (B) Effect of BCL2A1 knockdown on growth of M14 melanoma xenotransplants. Volume of tumors was determined 12 d after injection. Representative tumors from mice are shown for each shRNA. (C) Expression of HA-tagged BCL2A1 in pmel*BRAF(V600E) (21) detected by Western blotting. (D) BCL2A1 overexpression promotes soft-agar growth in oncogenic BRAF-transformed melanocytes. Cell number was determined after 2 wk.