Fig. 4.

HPLC analysis of total cellular ergosterol. (A) Chromatograms of ergosterol extracted from WT and set1Δ cells compared with commercially purified ergosterol. Ergosterol was detected at 280 nm, where peak elution was ∼9 min. (B) Relative amount of extracted ergosterol from the indicated strains compared with WT. Amounts of extracted ergosterol were determined by comparing extracted ergosterol to a standard curve of known concentrations of purified ergosterol. Purified cholesterol was added to each sample before extraction as an internal control. Analysis was performed using three biological replicates; error bars represent SEM.