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Effect of metal cofactors on enzyme activity and product formation of PcIDS1. (A) Different concentrations of Co2+, Mg2+, and Mn2+ were added to PcIDS1 and incubated with 50 μM IDP and 50 μM DMADP (n = 3, ±SD). (B) Different concentrations of Co2+, Mg2+, and Mn2+ were added to PcIDS1 and incubated with 50 μM IDP and 50 μM GDP (n = 3, ±SD).
