Table 1.

Kinetic constants for P. cochleariae PcIDS1 depend on the identity of both the metal cofactor and allylic substrate

Co2+					Mg2+
Fixed substrate	IDP(15 μM)	IDP(50 μM)	DMADP(50 μM)	GDP(50 μM)	IDP(50 μM)	IDP(50 μM)	DMADP(50 μM)	GDP(50 μM)
Substrate	DMADP	GDP	IDP	IDP	DMADP	GDP	IDP	IDP
Km (substrate), μM	11.60	24.31	0.84	1.46	∼1103	1.19	11.79	7.08
Vmax, μmol⋅min−1⋅mg−1 of protein	0.44	0.41	0.67	0.23	∼2.39	0.12	0.17	0.20
Ki (substrate), μM	n.d.	n.d.	46.62	n.d.	n.d.	1,047	572.7	322.1

n.d., not detectable.