Fig. 2.
Functional properties of heteromeric channels incorporating KV7.2 R6W or KV7.2 R6Q mutant subunits. (A) Macroscopic current traces from the indicated heteromeric channels in response to the indicated voltage protocols. Current scale, 200 pA; time scale, 0.1 s The arrows indicate the threshold voltage for current activation. (B and C) Conductance/voltage curves for the heteromeric channels formed by mutant subunits with KV7.2 and KV7.3 (B) or with KV7.2 (C). Continuous lines are Boltzmann fits to the experimental data. The cDNA ratios were: 1:1 for KV7.2+KV7.3, KV7.2+KV7.2 R6W, and KV7.2+KV7.2 R6Q, and 0.5:0.5:1 for KV7.2+KV7.2 R6W+KV7.3 and KV7.2+KV7.2 R6Q+KV7.3. Current scale, 200 pA; time scale, 100 ms.