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. 2012 Feb 7;110(11):E1035–E1044. doi: 10.1073/pnas.1219868110

Fig. 2.

Fig. 2.

Direct involvement of DprA in the control of comX expression. (A) Comparison of ssbB and comC expression in wild-type and dprA− cells reveals a strong influence of DprA on ssbB expression. Strains used: (Left) ssbB::luc R2018 (dprA−) and R1502; (Right) comC::luc R2017 (dprA−) and R1960. (B) Inactivation of dprA most strongly affects comX expression. PcomC, PcomX, and PssbB transcription was compared as described in Fig. 1. Strains used: (Top) comC::luc R2017 (dprA−) and R1960; (Middle) comX::luc R2240 and R2241 (both dprA−), and R2200 and R2218 (both dprA+); (Bottom) ssbB::luc R2018 (dprA−) and R1502. (C) Comparison of PcomX and PcomC transcription in wild-type cells with and without ectopic expression of dprA as an early com gene. Strains used: comC::luc R1998 (CEPE-dprA) and R1960 (Upper); comX::luc R2259 and R2260 (both CEPE-dprA+), and R2200 and R2218 (both without CEP) (Lower). See also Fig. S1C. (D) DprA inhibits PcomX transcription independently of any other late com gene product. Same experiment as in C but using comX− cells. Strains used: (Upper) comC::luc R2003 (CEPE-dprA) and R2002; (Lower) comX::luc R2265 (CEPE-dprA) and R2256.