. 2012 Feb 7;110(11):E1035–E1044. doi: 10.1073/pnas.1219868110

Table 1.

Synthetic lethality of dprA and cup mutations

Nature of the cup mutation^*	Mutation permissive for dprA inactivation^†	Mutation non permissive for dprA inactivation^‡
Point mutation	cup4 (comD^E214K), cup6 (comD^T290A), comD^D58E	trt1 (comD^D299N), cup3 (comD^T233I), cup5 (comD^Y312CK), cup11 (comD^Q226L), cup10 (comE^R120S),

Potential dprA::spc (Spc^R) transformants appeared only after prolonged incubation (41 h) as microhemolysis zones (e.g., Fig. S3A), which did not grow further and could not be recovered for growth in liquid medium.

*These mutations have been previously described (21).

^†Frequency of dprA::spc transformants with normal colony size of ∼0.2 relative to the rpsL41 (Sm^R) reference marker (i.e., close to the expected value).

^‡Approximately 3 log reduction in the frequency of dprA::spctransformants with normal colony size (e.g., Fig. S3A) compared with the permissive situation.