Fig. 1.

Characterization of dividing cells in the V-SVZ. Confocal analysis of whole mounts of the lateral wall of the lateral ventricle with examples of cells stained for different markers. In hGFAP::GFP mice, the majority of GFP⁺ cells were stained for GFAP (A). A small fraction of cells expressed both GFP and Ascl1 (B); for quantifications, Results and J and K). Also, a small fraction of Ascl1⁺ cells expressed DCX (C). Thirty minutes (') after EdU injection, a small fraction of EdU⁺ cells expressed GFP (in hGFAP::GFP mice (D), whereas the majority expressed Ascl1 (E) or DCX (F); for quantification, see Results and L. Consistently, the fraction of GFP⁺ cells (in hGFAP::GFP mice) labeled by mitotic marker pHH3 was small (G). Most of the pHH3⁺ cells were either Ascl1⁺ (H) or DCX⁺ (I); for quantification, see Results and M. (Scale bars: 50 μm.) Arrows indicate double-labeled cells; arrowheads indicate single-labeled cells. (L–M) A fraction (labeled “?”) of EdU⁺ or pHH3⁺ did not express GFP (B1 cells), Ascl1(C cells), or DCX (A cells) markers. (D–F and L) Thirty minutes after EdU injection. Means (n = 3) ± SEM.