Fig. 4.

Length of cell cycle phases for DCX⁺ cells. (A and B) DA method; for experimental outline, see Fig. 2F. Confocal analysis at 3.5 h (triple-labeled cell; arrow). (B) T_S and T_C determined by DA. (C–E) Short PLM method (for experimental outline see Fig. 3G). (C) Confocal analysis 3 h after CldU (triple-labeled cell; arrow). (D) Ratio of triple-labeled cells (DCX⁺pHH3⁺CldU⁺) to mitotic DCX⁺ cells. (E) T_G2 and T_M quantified by PLM Least square fit; line a, y = 0.268 × −0.168; R² = 0.985. The CL method and the long version of PLM cannot be reliably used for DCX⁺ cells because of their active tangential migration (Results). (Scale bars: 25 μm.)