Table 1.

Names and amounts of probe substrates in microsomal incubations.

Isoform	Substrate	Metabolite	Ranges of substrate concentrations in the incubations, µM	Fluorescence detection excitation/emission
CYP1A	ER	resorufin	0.1–12.0	560/586 nm
CYP2A	coumarin	hydroxycoumarin	2.5–300.0	338/458 nm
CYP3A	BFC	HFC	0.4–120.0	410/538 nm

ER, 7-ethoxyresorufin; BFC, 7-benzyloxy-4-trifluoromethylcoumarin

HFC, 7-hydroxy-7-benzyloxy-trifluoromethylcoumarine