Table 2.

Mutagenic activity for aqueous extract of C. nervosum based on non-metabolic (–S9) and metabolic activation (+S9) using S. typhimurium TA 98 and TA100 strain.

Treatment	Average of His+ revertant colonies
	TA 98		TA100
	– S9	+ S9	– S9	+ S9
AF-2 2 ug/ml	286±32.6	N.A.	834±184.3	N.A.
2-AA 10 ug/ml	N.A.	408±35.9	N.A.	612±186.9
DW 50 ul	32±6.3	37±9.4	143±34.8	180±36.7
C. nervosum extract 0.8 mg/ml	32±6.1	26±3.7	138±39.2	134±36.1
C. nervosum extract 4 mg/ml	32±9.0	24±3.7	137±41.7	135±38.0
C. nervosum extract 20 mg/ml	32±8.8	22±4.8	133±31.4	139±36.6
C. nervosum extract 100 mg/ml	31±8.0	24±1.7	141±44.2	129±32.2
C. nervosum extract 200 mg/ml	37±15	30±9.1	N.A.	N.A.

The His⁺ revertant colonies are expressed as Mean±SEM of three independent replicates.

AF-2; 2-(2-furyl)-3-(5-nitro-2-furyl)-acrylamide, 2-AA; 2-aminoanthracene, DW; distilled water, N.A.; not analyzed