Table 1.
Effect of GIE on liver TBARS, GSH, SOD, CAT, GPx, GR and hTG in ETOH- intoxicated rats.
| Biochemical parameters | Group I Normal Control distilled water | Group II Toxicant Control ETOH (5 g/kg, 20% w/v) | Group III GIE (400 mg/kg) | Group IV GIE (800 mg/kg) | Group V Silymarin (200 mg/kg) |
|---|---|---|---|---|---|
| TBARS (nmol MDA/g wet tissue) | 34.72±4.07 | 59.31±7.32b | 50.69±1.01 | 39.86±1.62x | 34.89±1.77y |
| GSH (mol/g wet tissue) | 1.33±0.03 | 1.04±0.02a | 1.08±0.01 | 1.15±0.02x | 1.19±0.02y |
| SOD (U/mg protein) | 43.57±0.86 | 32.60±1.55a | 36.61±2.35 | 42.23±1.81y | 43.55±0.84z |
| CAT (U/mg protein) | 5.13±1.05 | 1.50±0.28b | 3.11±0.11 | 4.69±0.37y | 5.02±0.71y |
| GPx (U/mg protein) | 2.5±0.32 | 1.00±0.27a | 1.96±0.06x | 2.28±0.13y | 2.4±0.19y |
| GR (U/mg protein) | 798.32±35.44 | 256.15±61.89a | 437.38±28.30 | 467.03±75.98x | 576.25±31.58y |
| HTG (mg/dL) | 2.37±0.30 | 13.21±1.01a | 6.07±0.57z | 4.40±0.24z | 2.85±0.33z |
Values are mean ± SEM for 6 animals in each group
a
p-values: <0.001
b
<0.01
c
<0.05 when Toxicant Control compared with Normal Control
x
<0.05
y
<0.01
z
<0.001 when Experimental groups compared with Toxicant Control
1 unit of CAT = µmol H2O2 consumed / min / mg protein
1 unit of GPx = µg GSH utilized / min / mg protein
1 unit of GR = nmol NADPH oxidized / min / mg protein